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Research
TISSUE ENGINEERING & BIOMATERIALS 組織工程與生醫材料
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1. Cell Therapy using 3D Stem Cell Spheroids
建立三維幹細胞球體以進行細胞治療
3D Stem Cell Spheroid
“Transplantation of 3D MSC/HUVEC spheroids with neuroprotective and proangiogenic potentials ameliorates ischemic stroke brain injury,” Biomaterials, vol. 272, 120765, 2021.
“Injection of hybrid 3D spheroids composed of podocytes, mesenchymal stem cells, and vascular endothelial cells into the renal cortex improves kidney function and replenishes glomerular podocytes,” Bioengineering & Translational Medicine, e10212, 2021.
Cell Therapy for Treating Ischemic Stroke
Transplantation of 3D MSC/HUVEC spheroids with neuroprotective and proangiogenic potentials ameliorates ischemic stroke brain injury, Biomaterials, vol. 272, 120765, 2021.
將幹細胞以三維球體形式移植至小鼠腦中風區域後，可有效提昇移植細胞的存活率，並降低局部發炎反應、達到神經保護與促進神經/血管新生等功能，並使小鼠中風後的運動能力得以有顯著改善。
Cell Therapy for Treating Kidney Injury, Peripheral Nerve Injury, and Type I Diabetes Mellitus
Injection of hybrid 3D spheroids composed of podocytes, mesenchymal stem cells, and vascular endothelial cells into the renal cortex improves kidney function and replenishes glomerular podocytes, Bioengineering & Translational Medicine, e10212, 2021.
Development of a novel hanging drop platform for engineering controllable 3D microenvironments, Frontiers in Cell and Developmental Biology, vol. 8, 327, 2020.
3D Spheroids of umbilical cord blood MSC-derived Schwann cells promote peripheral nerve regeneration, Frontiers in Cell and Developmental Biology, vol. 8, 604946, 2020.
Enhancement of subcutaneously transplanted β cell survival using 3D stem cell spheroids with pro-angiogenic & pro-survival potential, Advanced Biosystems, vol. 4, 1900254, 2020
足細胞(podocyte)在腎絲球的過濾作用中扮演重要角色，但成人的足細胞不具有再生能力，因此當腎臟受到藥物、疾病或創傷等因素造成足細胞數量減少時，將嚴重影響腎臟功能。本研究嘗試以細胞療法進行外源性足細胞的移植，並進一步結合血管內皮細胞與間葉幹細胞，探討三種細胞間的交互作用。
胰島β細胞移植可使部分第一型糖尿病患者恢復自身調節血糖的能力；然而如何有效改善移植細胞的存活率，仍是尚待解決的問題。藉由將幹細胞組裝成為三維球體的形式，可使得幹細胞促進血管新生的能力得以顯著上升。因此將三維幹細胞球體與胰島β細胞一起進行移植，可有效增加β細胞的存活率，使治療效果進一步提昇。
2. Functional Biomaterials for Regenerative Medicine
功能性生醫材料於再生醫學之應用
Cell-derived ECM for Regenerative Medicine
Bioactive decellularized extracellular matrix derived from 3D stem cell spheroids under macromolecular crowding serves as a scaffold for tissue engineering, Advanced Healthcare Materials, vol. 10, 2100024, 2021.
將三維幹細胞球體進行去細胞處理後的基質中，含有豐富的生物活性分子，可做為組織工程支架。
Artificial Corneal Stroma for Supporting
Corneal Endothelial Cell Transplantation
Gelatin scaffold with multifunctional curcumin-loaded lipid-PLGA hybrid microparticles for regenerating corneal endothelium, Materials Science and Engineering C: Materials for Biological Applications, vol. 120, 111753, 2021.
本研究擬開發新型角膜組織工程支架，用以培養角膜內皮細胞，並用於細胞移植。藉由導入微米形式之藥物制放載體，於移植同時輸送抗氧化、抗細胞凋亡之藥物，或能有效提昇移植後的角膜內皮細胞存活率，進而提昇移植成功率。
Oxygen-generating Materials for Tissue Engineering
Optimizing an injectable Composite oxygen-generating system for relieving tissue hypoxia, Frontiers in Bioengineering and Biotechnology, vol. 8, 511, 2020.
氧氣是細胞生存的必要條件之一。當氧氣供應不足而形成缺氧的微環境時，將不利於細胞的存活。本研究期望開發可產生氧氣之載體，並藉其增加局部區域的氧氣含量，改善缺氧區域的細胞存活率及其功能。
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