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Research
TISSUE ENGINEERING & BIOMATERIALS 組織工程與生醫材料
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1. Cell Therapy using 3D Stem Cell Spheroids
建立三維幹細胞球體以進行細胞治療
3D Stem Cell Spheroid
Stem cell therapy has demonstrated significant potential in regenerative medicine, yet traditional cell transplantation methods often suffer from low delivery efficiency, resulting in considerable cell loss during administration. By pre-assembling stem cells into three-dimensional spheroids, we have markedly enhanced delivery efficiency and overcome this critical challenge. Our research further indicates that stem cells organized in 3D spheroids exhibit superior therapeutic capabilities. In various animal models, we have confirmed that transplanting stem cells in this spheroid form amplifies their regenerative potential, with successful applications in treating conditions such as ischemic stroke, peripheral nerve injury, kidney injury, tendon rupture, type I diabetes, and corneal damage.
“Assembly of MSCs into a spheroid configuration increases poly(I:C)-mediated TLR3 activation and the immunomodulatory potential of MSCs for alleviating murine colitis,” Stem Cell Research & Therapy, vol. 16, pp. 172, 2025.
“Schwann cells acquire a repair phenotype after assembling into spheroids and show enhanced in vivo therapeutic potential for promoting peripheral nerve repair,” Bioengineering & Translational Medicine, vol. 9, pp.e10635, 2024.
“The transplantation of 3-dimensional spheroids of adipose-derived stem cells promotes Achilles tendon healing in rabbits by enhancing the proliferation of tenocytes and suppressing M1 macrophages,” American Journal of Sports Medicine, vol. 52, pp.406-422, 2024.
“Transplantation of 3D MSC/HUVEC spheroids with neuroprotective and proangiogenic potentials ameliorates ischemic stroke brain injury,” Biomaterials, vol. 272, 120765, 2021.
“Injection of hybrid 3D spheroids composed of podocytes, mesenchymal stem cells, and vascular endothelial cells into the renal cortex improves kidney function and replenishes glomerular podocytes,” Bioengineering & Translational Medicine, e10212, 2021.
2. Decellularized 3D ECM derived from Stem Cell Spheroids as
a Bioactive Material for Regenerative Medicine
三維幹細胞球體衍生基質於再生醫學之應用
Cell-derived ECM for Regenerative Medicine
We assemble stem cells into spheroids and subsequently subject them to decellularization, resulting in three-dimensional decellularized extracellular matrices (3D dECM). These matrices serve as both physical scaffolds for tissue engineering and bioactive platforms due to the retention of the stem cell secretome, thereby exhibiting remarkable therapeutic potential.
“Tailoring the therapeutic potential of stem cell spheroid-derived decellularized ECM through post-decellularization BDNF incorporation to enhance brain repair,” Biomaterials, vol. 321, pp. 123332, 2025.
“Implantation of MSC spheroid-derived 3D decellularized ECM enriched with the MSC secretome ameliorates
traumatic brain injury and promotes brain repair,” Biomaterials, vol. 315, pp. 122941, 2025.
“Bioactive decellularized extracellular matrix derived from 3D stem cell spheroids under macromolecular crowding serves as a scaffold for tissue engineering,” Advanced Healthcare Materials, vol. 10, 2100024, 2021.
3. Functional Biomaterials for Regenerative Medicine
功能性生醫材料於再生醫學之應用
At tissue lesion sites, the microenvironment is often characterized by ischemia, oxygen and nutrient deprivation, inflammation, and high oxidative stress—conditions that impede both endogenous regenerative mechanisms and the efficacy of transplanted cells. To address these challenges, we employ polymer-based biomaterials to create controlled release systems that deliver oxygen, glucose, or anti-inflammatory agents. By specifically targeting these adverse conditions, our systems accelerate tissue regeneration and reduce oxidative and inflammatory stress. Improving the tissue microenvironment in this way enhances the survival rates of both endogenous and transplanted cells, ultimately leading to better clinical outcomes.
“Injectable glucose-releasing microgels enhance the survival and therapeutic potential of transplanted MSCs under ischemic conditions,” Advanced Healthcare Materials, vol. 14, pp. 2401724, 2025.
“Strontium peroxide-loaded composite scaffolds capable of generating oxygen and modulating behaviors of osteoblasts and osteoclasts,” International Journal of Molecular Science, vol. 23, 6322, 2022.
“Gelatin scaffold with multifunctional curcumin-loaded lipid-PLGA hybrid microparticles for regenerating corneal endothelium,” Materials Science and Engineering C: Materials for Biological Applications, vol. 120, 111753, 2021.
“Optimizing an injectable Composite oxygen-generating system for relieving tissue hypoxia,” Frontiers in Bioengineering and Biotechnology, vol. 8, 511, 2020.
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